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The Journal of nutrition 膳食脂肪和糖差异影响高热量喂养 C57BL/6 雄性小鼠心脏 ERK 和 AKT 通路的 β-肾上腺素能刺激。
影响因子:4.687 DOI:10.1093/jn/nxz342
作者: Ashraf S,Yilmaz G,Chen X,Harmancey R 发表时间:2020-02-04 13:29:31
keywords: Ashraf SYilmaz GChen XHarmancey R
关键词: 西方饮食 心脏肥大 高脂饮食 高糖饮食 胰岛素信号 蛋白质合成 蔗糖
Abstract
BACKGROUND:High dietary fat and sugar promote cardiac hypertrophy independently from an increase in blood pressure. The respective contribution that each macronutrient exerts on cardiac growth signaling pathways remains unclear.,OBJECTIVE:The goal of this study was to investigate the mechanisms by which high amounts of dietary fat and sugar affect cardiac growth regulatory pathways.,METHODS:Male C57BL/6 mice (9 wk old; n = 20/group) were fed a standard rodent diet (STD; kcal% protein-fat-carbohydrate, 29-17-54), a high-fat diet (HFD; 20-60-20), a high-fat and high-sugar Western diet (WD; 20-45-35), a high-sugar diet with mixed carbohydrates (HCD; 20-10-70), or a high-sucrose diet (HSD; 20-10-70). Body composition was assessed weekly by EchoMRI. Whole-body glucose utilization was assessed with an intraperitoneal glucose tolerance test. After 6 wk on diets, mice were treated with saline or 20 mg/kg isoproterenol (ISO), and the activity of cardiac growth regulatory pathways was analyzed by immunoblotting. Data were analyzed by ANOVA with data from the STD group included for references only.,RESULTS:Compared with HCD and HSD, WD and HFD increased body fat mass 2.7- to 3.8-fold (P < 0.001), induced glucose intolerance (P < 0.001), and increased insulin concentrations >1.5-fold (P < 0.05), thereby enhancing basal and ISO-stimulated AKT phosphorylation at both threonine 308 and serine 473 residues (+25-63%; P < 0.05). Compared with HFD, the high-sugar diets potentiated ISO-mediated stimulation of the glucose-sensitive kinases PYK2 (>47%; P < 0.05 for HCD and HSD) and ERK (>34%; P < 0.05 for WD, HCD, and HSD), thereby leading to increased phosphorylation of protein synthesis regulator S6K1 at threonine 389 residue (>64%; P < 0.05 for WD, HCD, and HSD).,CONCLUSIONS:Dietary fat and sugar affect cardiac growth signaling pathways in C57BL/6 mice through distinct and additive mechanisms. The findings may provide new insights into the role of overnutrition in pathological cardiac remodeling.
摘 要
背景: 高脂肪和高糖饮食可独立于血压升高而促进心肌肥厚。每种宏量营养素对心脏生长信号通路的各自贡献仍不清楚。, 目的: 本研究的目的是探讨高量膳食脂肪和糖影响心脏生长调控途径的机制。, 方法: 雄性 C57BL/6 小鼠 (9 周龄; n = 20/组) 喂食标准啮齿类动物饮食 (STD; kcal % 蛋白质-脂肪-碳水化合物,29-17-54) 、高脂饮食 (HFD; 20-60-20) 、高脂高糖西方饮食 (WD; 20-45-35) 、混合碳水化合物的高糖饮食 (HCD; 20-10-70) 或高蔗糖饮食 (HSD; 20-10-70)。每周通过 EchoMRI 评估身体成分。通过腹腔内葡萄糖耐量试验评估全身葡萄糖利用。饮食 6 周后,用生理盐水或 20 mg/kg 异丙肾上腺素 (ISO) 处理小鼠,通过免疫印迹分析心脏生长调节通路的活性。通过 ANOVA 分析数据,包括来自 STD 组的数据仅供参考。,结果: 与 HCD 和 HSD 相比,WD 和 HFD 的体脂量增加 2.7-3.8 倍 (P
期刊介绍
《JOURNAL OF NUTRITION》 (点击进入期刊详情)
英文简介 : The premier journal in its field, The Journal of Nutrition continues to have as its goal the publication of papers based on original nutrition research in humans and other animal species. In circulation since 1928, it is the official monthly publication of the American Society for Nutritional Sciences. It is distributed to more than 4,500 subscribers worldwide. The Journal publishes original research reported by authors in over 30 countries. To provide expert evaluation of the many areas of nutrition research, the editorial office is assisted by scientists who serve as Associate Editors, Regional Correspondents, editorial board members and ad-hoc reviewers chosen for their nutritional science expertise. They provide constructive evaluation and fair and rapid editorial processing. Reports of research on the following and other areas of nutrition research are published: Animal Nutrition, Carbohydrates and Fibers, Community and International Nutrition, Diet and Disease, Energy and Macronutrient Metabolism, Growth and Development, Human Nutrition, Lipids, Maternal and Infant Nutrition, Metabolism and Hormonal Regulation, Neuroscience and Nutrition, Nutrient-Gene Interactions, Nutrient Metabolism, Nutrition and Immunology, Nutrition, Pharmacology and Toxicology, Nutritional Epidemiology, Proteins and Amino Acids, Public Health Nutrition and Policy, Vitamins and Minerals.
中文简介 : (来自Google、百度翻译) 《营养学杂志》是该领域的首要期刊,其目标仍然是发表基于人类和其他动物物种的原始营养学研究的论文。自1928年起发行,它是美国营养科学学会的官方月刊。它被分发到全球4500多个用户。该杂志发表了30多个国家的作者所报道的原始研究。为了对营养研究的许多领域进行专家评估,编辑部由科学家协助,这些科学家担任副编辑、区域通讯员、编辑委员会成员以及因其营养科学专业知识而选定的特设评审员。他们提供建设性的评估和公平、快速的编辑处理。发表了以下和其他营养研究领域的研究报告:动物营养、碳水化合物和纤维、社区和国际营养、饮食和疾病、能量和大营养代谢、生长和发育、人类营养、脂类、母婴营养、代谢和激素调节、神经系统和神经系统。科学与营养、营养基因相互作用、营养代谢、营养与免疫学、营养、药理学与毒理学、营养流行病学、蛋白质与氨基酸、公共卫生营养与政策、维生素与矿物质。
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NUTRITION & DIETETICS (营养学)

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