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The Journal of nutrition 急性高能量、高脂喂养增加健康男性循环 FGF21 、 LECT2 和胎球蛋白-A。
影响因子:4.687 DOI:10.1093/jn/nxz333
作者: Willis SA,Sargeant JA,Yates T,Takamura T,Takayama H,Gupta V,Brittain E,Crawford J,Parry SA,Thackray AE,Varela-Mato V,Stensel DJ,Woods RM,Hulston CJ,Aithal GP,King JA 发表时间:2020-02-05 02:10:13
keywords: Willis SASargeant JAYates TTakamura TTakayama HGupta VBrittain ECrawford JParry SAThackray AEVarela-Mato VStensel DJWoods RMHulston CJAithal GPKing JA
关键词: FGF21 LECT2 胎球蛋白A 胎球蛋白B 肝因子 高脂饮食 胰岛素抵抗 过度馈电 硒蛋白P
Abstract
BACKGROUND:Hepatokines such as fibroblast growth factor 21 (FGF21), leukocyte cell-derived chemotaxin 2 (LECT2), fetuin-A, fetuin-B, and selenoprotein P (SeP) are liver-derived proteins that are modulated by chronic energy status and metabolic disease. Emerging data from rodent and cell models indicate that hepatokines may be sensitive to acute nutritional manipulation; however, data in humans are lacking. OBJECTIVE:The aim was to investigate the influence of hyperenergetic, high-fat feeding on circulating hepatokine concentrations, including the time course of responses. METHODS:In a randomized, crossover design, 12 healthy men [mean ± SD: age, 24 ± 4 y; BMI (kg/m2), 24.1 ± 1.5] consumed a 7-d hyperenergetic, high-fat diet [HE-HFD; +50% energy, 65% total energy as fat (32% saturated, 26% monounsaturated, 8% polyunsaturated)] and control diet (36% total energy as fat), separated by 3 wk. Whole-body insulin sensitivity was assessed before and after each diet using oral-glucose-tolerance tests. Fasting plasma concentrations of FGF21 (primary outcome), LECT2, fetuin-A, fetuin-B, SeP, and related metabolites were measured after 1, 3, and 7 d of each diet. Hepatokine responses were analyzed using 2-factor repeated-measures ANOVA and subsequent pairwise comparisons. RESULTS:Compared with the control, the HE-HFD increased circulating FGF21 at 1 d (105%) and 3 d (121%; P ≤ 0.040), LECT2 at 3 d (17%) and 7 d (32%; P ≤ 0.004), and fetuin-A at 7 d (7%; P = 0.028). Plasma fetuin-B and SeP did not respond to the HE-HFD. Whole-body insulin sensitivity was reduced after the HE-HFD by 31% (P = 0.021). CONCLUSIONS:Acute high-fat overfeeding augments circulating concentrations of FGF21, LECT2, and fetuin-A in healthy men. Notably, the time course of response varies between proteins and is transient for FGF21. These findings provide further insight into the nutritional regulation of hepatokines in humans and their interaction with metabolic homeostasis. This study was registered at clinicaltrials.gov as NCT03369145.
摘 要
背景: 肝因子如成纤维细胞生长因子 21 (FGF21) 、白细胞衍生趋化因子 2 (LECT2) 、胎球蛋白-A 、胎球蛋白-B 和硒蛋白 P (SeP) 是由慢性能量状态和代谢性疾病调节的肝源性蛋白。来自啮齿类动物和细胞模型的新数据表明,肝因子可能对急性营养操作敏感; 然而,缺乏人类的数据。 目的: 旨在研究高能量、高脂喂养对循环肝因子浓度的影响,包括反应的时间过程。 方法: 在一项随机、交叉设计中,12 名健康男性 [平均值 ± SD: 年龄,24 ± 4 y; BMI (kg/m2),24.1 ± 1.5] 消耗了 7-d 的高能量, 高脂饮食 [HE-HFD; + 50% 能量,65% 总能量作为脂肪 (32% 饱和,26% 单不饱和,8% 多不饱和)]和控制饮食 (36% 总能量为脂肪),分离 3 wk。采用口服葡萄糖耐量试验在每次饮食前后评估全身胰岛素敏感性。每次饮食 1 、 3 和 7 d 后测定 FGF21 (主要结局) 、 LECT2 、胎球蛋白-A 、胎球蛋白-B 、 SeP 和相关代谢物的空腹血浆浓度。使用 2 因子重复测量 ANOVA 和随后的成对比较分析肝脏病因子反应。 结果: 与对照组相比,HE-HFD 升高循环 FGF21 在 1 d (105%) 和 3 d (121%; P ≤ 0.040),LECT2 在 3 d (17%) 和 7 d (32%; P ≤ 0.004),和 7 d 时胎球蛋白-A (7%; P = 0.028)。血浆胎球蛋白-B 和 SeP 对 HE-HFD 无反应。HE-HFD 后全身胰岛素敏感性降低 31% (P = 0.021)。 结论: 急性高脂过量喂养可增加健康男性 FGF21 、 LECT2 和胎球蛋白-A 的循环浓度。值得注意的是,fgf21 的反应时程因蛋白而异,是短暂的。这些发现为人类肝因子的营养调节及其与代谢稳态的相互作用提供了进一步的见解。本研究在 clinicaltrials.gov 上注册为 nct03369 145。
期刊介绍
《JOURNAL OF NUTRITION》 (点击进入期刊详情)
英文简介 : The premier journal in its field, The Journal of Nutrition continues to have as its goal the publication of papers based on original nutrition research in humans and other animal species. In circulation since 1928, it is the official monthly publication of the American Society for Nutritional Sciences. It is distributed to more than 4,500 subscribers worldwide. The Journal publishes original research reported by authors in over 30 countries. To provide expert evaluation of the many areas of nutrition research, the editorial office is assisted by scientists who serve as Associate Editors, Regional Correspondents, editorial board members and ad-hoc reviewers chosen for their nutritional science expertise. They provide constructive evaluation and fair and rapid editorial processing. Reports of research on the following and other areas of nutrition research are published: Animal Nutrition, Carbohydrates and Fibers, Community and International Nutrition, Diet and Disease, Energy and Macronutrient Metabolism, Growth and Development, Human Nutrition, Lipids, Maternal and Infant Nutrition, Metabolism and Hormonal Regulation, Neuroscience and Nutrition, Nutrient-Gene Interactions, Nutrient Metabolism, Nutrition and Immunology, Nutrition, Pharmacology and Toxicology, Nutritional Epidemiology, Proteins and Amino Acids, Public Health Nutrition and Policy, Vitamins and Minerals.
中文简介 : (来自Google、百度翻译) 《营养学杂志》是该领域的首要期刊,其目标仍然是发表基于人类和其他动物物种的原始营养学研究的论文。自1928年起发行,它是美国营养科学学会的官方月刊。它被分发到全球4500多个用户。该杂志发表了30多个国家的作者所报道的原始研究。为了对营养研究的许多领域进行专家评估,编辑部由科学家协助,这些科学家担任副编辑、区域通讯员、编辑委员会成员以及因其营养科学专业知识而选定的特设评审员。他们提供建设性的评估和公平、快速的编辑处理。发表了以下和其他营养研究领域的研究报告:动物营养、碳水化合物和纤维、社区和国际营养、饮食和疾病、能量和大营养代谢、生长和发育、人类营养、脂类、母婴营养、代谢和激素调节、神经系统和神经系统。科学与营养、营养基因相互作用、营养代谢、营养与免疫学、营养、药理学与毒理学、营养流行病学、蛋白质与氨基酸、公共卫生营养与政策、维生素与矿物质。
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