急性桐油中毒导致大鼠心肌损伤的酶组织化学及超微结构观察

目的：探讨急性桐油中毒对心肌的损伤机制。方法：模拟人体桐油中毒方式灌喂大鼠，复制大鼠急性桐油中毒模型。采用光镜，电镜及组织化学方法，观察急性桐油中毒对大鼠心肌的影响。结果：急性桐油中毒心肌明显受损，光镜下细胞及间质水肿，电镜下心肌线粒体肿胀，空泡样变，并有髓样小体形成。组化观察心肌琥珀酸脱氢酶(SDH)活性显著下降，乳酸脱氢酶(LDH)活性有增强趋势。结论：急性桐油中毒通过对心肌细胞线粒体的直接损伤以及对能量代谢的影响而引起心脏功能的明显损害。

普伐他汀和西立伐他汀对高胆固醇喂养兔的血管内皮功能的影响

目的：观察普伐他汀和西立伐他汀对高胆固醇喂养兔的血管内皮功能的影响。方法：28只纯种日本大耳白兔，随机分为4组(每组7只)：高胆固醇对照(HC)组喂以1.5％的高胆固醇饲料共8周，普伐他汀加高胆固醇(P+HC)组和西立伐他汀加高胆固醇(C+HC)组均先喂以1.5％的高胆固醇饲料4周，之后P+HC组继续喂以1.5％的高胆固醇饲料加普伐他汀10 mg·kg-1·d-14周，C+HC组喂以1.5％的高胆固醇饲料加西立伐他汀0．3 mg·kg-1·d-14周，正常对照组(NC组)喂以普通饲料共8周。8周后于麻醉情况下取血测定血脂，并分离胸主动脉去除脂肪和结缔组织，切成4 mm长的血管环置浴槽中做离体灌流实验，分别观察对乙酰胆碱、硝酸甘油和去甲肾上腺素的剂量反应曲线。结果：HC组血清胆固醇水平明显高于NC组，P+HC组和C+HC组则显著低于HC组。HC组血管对乙酰胆碱的内皮依赖性舒张反应明显弱于NC组，且对去甲肾上腺素的收缩反应高于NC组；而P+HC组和C+HC组这些反应接近NC组，它们之间无显著差异。4组血管对硝酸甘油的非内皮依赖性舒张反应相似。结论：普伐他汀和西立伐他汀均可预防高胆固醇喂养兔的血管内皮功能失调，二者之间无显著差异。

川芎嗪对实验性肾炎肾皮质内血小板活化因子、血栓素B2的影响

目的：探讨川芎嗪对实验性肾炎肾皮质内血小板活化因子(PAF)、血栓素B2(TXB2)水平的影响。方法：复制大鼠加速型抗肾小球基膜(GBM)肾炎模型。分为肾炎组、肾炎+川芎嗪组(治疗组)。结果：治疗组各期尿蛋白量较肾炎组减少(P<0.05，P<0.01)，第21 d时血清肌酐含量低于肾炎组(P<0.05)，光镜和电镜下肾脏病理改变较肾炎组为轻，同时肾皮质内PAF、TXB2含量少于肾炎组(P<0.01)。结论：川芎嗪治疗实验性肾炎有效，其机理可能与减少肾皮质内PAF、TXB2等有关。

呼吸性酸碱失衡内髓集合管细胞培养模型的制备

缺血／再灌注损伤时丝裂原活化蛋白激酶信号转导通路的变化

Mitogen - activated protein kinases (MAPKs), including extracellular signal - regulated kinases (ERKs), c - Jun NH2 - terminal kinases (JNKs) and p38 MAPK, play an important role in transducting environmental stimuli to the transcriptional machinery in the nucleus in mammalian cells by virtue of their ability to phosphorylate and regulate the activity of various transcription factors. It was recently found that the changes in activity of MAPKs occurred during ischemia/reperfusion, but the biological significance of the changes was still controversial.

长时程增强与长时程抑制的研究

The impairment of learning and memory function in the central nervous system(CNS)is one of the main features of aging and Alzheimer' s disease (AD). Many experimental results have showed that long- term memory(LTM) is related to long- term potentiation(LTP) and long- term depression (LTD). They intluenee each other. The relationship between LTP and LTD is complex. Therefore, it is important to study the learning and memory mechanism from LTP、LTD and gene expression in the CNS.

Fractalkine和CX3CR1-新发现的一对与白细胞趋化和粘附功能密切相关的分子

Fractalkine has been recognized as the first member of the fourth chemokine family recently and its receptor, CX3CR1, was identified afterwards. This pair of ligand/receptor play important roles in the migration and adhesion of peripheral leukocyte through different ways. They are also likely to be related to the embryogenesis and regeneration of several organs and may possess some functions in the central nervous system.

类P3L与痴呆动物模型的确立